Role of direct antiglobulin test in assessing the severity of extravascular hemolysis in autoimmune hemolytic anemia – analysis from a cross-sectional study

Background: Autoimmune hemolytic anemia (AIHA) is an immune disease characterized by hemolysis and which results from increased red cell destruction due to auto antibodies directed against self-antigens on cells. Clinical characteristics potential associated in AIHA can greatly depend the serological characteristics. Aims Objectives: This study was done find out association of Grade polyspecific Direct Antiglobulin test (DAT), complement involvement immunoglobulin G (IgG) subclass with severity patients. Materials Methods: a cross-sectional conducted for period 18 months March 1, 2012, August 31, 2013. Fifty consecutive patients diagnosed positive DAT EDTA anticoagulated blood samples using poly specific anti human globulin reagent were followed up various immunohematological tests such as monospecific DAT, IgG subtyping, thermal amplitude performed. Hematological biochemical parameters hemoglobin, percentage reticulocyte, total serum bilirubin, lactate dehydrogenase also recorded. Data analyzed SPSS v17. Results: Majority belonging 31–40 year age group there remarkable female predilection. Secondary more common (56%) than primary. SLE identified most underlying disorder secondary AIHA. (46%) 4 positive. Predominant autoantibody (52%). Of cases, IgG1 predominant subclass. C3d alone detected 18% cases together 30%. About 48% our warm type, 32% mixed 20% cold type. 80% population treated steroids alone. A small (16%) needed immunosuppressant addition still smaller (4%) splenectomy. Higher grades positivity severe degree hyperbilirubinemia. The demonstrated correlation between grade hemolysis. Conclusion: this predominantly Warm involvement. significant found higher jaundice group. exists DCT well.